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Three replicates of each sample were measured and corrected with an ultrapure water (Milli-Q) Fosinopril Sodium (Monopril)- FDA at подробнее на этой странице temperature.

Before all measurements, samples as well as an ultrapure water (Milli-Q) blank were allowed to warm up to room temperature. From the 14 samples measured, the number of fluorophores was determined by a split-half validation and Fosinopril Sodium (Monopril)- FDA residual analysis (Reference Stedmon and BroStedmon and Bro, 2008).

Then the enrichment factor, Dcfor DOM was calculated as where subscripts i источник статьи w refer to ice and water, respectively (modified after Weeks and Ackley, 1982). In Equation (3), the identical behaviour of DOM and salinity results in an enrichment factor of 0. For Dc of natural samples of nat08, ice samples were related to the under-ice water samples on the sampling day.

No Dc value is calculated for nat07, because of missing salinity values in the under-ice water. LC-SEC was performed using a silica-based TSK G3000SWxl column (7. Нажмите чтобы перейти Fosinopril Sodium (Monopril)- FDA buffer (3. A baseline correction was done for all samples by normalizing the chromatogram to the value at 12 min.

Fosinopril Sodium (Monopril)- FDA this study, the retention time, R t (min), between 12 and 20 min was used directly to describe the molecular size distribution of DOM. In LC-SEC, molecules with large molecular mass have short retention times, and the retention time increases for molecules with decreasing molecular mass. Prior to LC-SEC analysis, the salinities of under-ice water samples of exp08 and nat08 were adjusted with Milli-Q water to 1, equivalent to the salinity found in the ice samples of exp08.

In order to quantify the behaviour of DOM in Baltic Sea ice during initial freezing, a short-term experiment was carried out (exp07). These results show that a CDOM,350 was enriched relative to salinity in ice, but in water, a CDOM,350 behaved conservatively. Error bars indicate the standard deviation between replicates (a). Characterization of samples in Fosinopril Sodium (Monopril)- FDA of sample type (experimental or natural ice or under-ice and initial water), sampling site and salinities.

After 12 hours of ice продолжить чтение, the a CDOM,350 of ice was again lower than in the under-ice water (Fig. We also studied the behaviour of DOM during a longer period of ice growth (exp08). Again, Dc values showed that CDOM was enriched relative to salinity in ice, but not in under-ice water (Fig. The quantitative enrichment of DOM was additionally assessed by examining the fluorophoric DOM in natural (nat08) and experimentally grown ice (exp08).

For these samples, the PARAFAC analysis identified three fluorophores. Fluorophore 1 (C1) had excitation maxima at 240 and 340 nm, with the emission maximum at 484 nm (Fig.

Fluorophore 2 (C2) had excitation maxima at 240 and 305 nm, with the emission maximum at 404 nm. Fluorophore C3 Fosinopril Sodium (Monopril)- FDA excitation maxima at 240 and 280 nm, with emission maximum at 340 nm. PARAFAC modelled fluorescent components of experimentally and naturally grown ice (exp08, nat08).

For both нажмите для продолжения, three fluorescent components were identified by PARAFAC modelling. For each fluorophore the maximal fluorescence intensity was used to calculate Dc values (Equation (3)).

In both naturally (nat08) and experimentally grown ice посмотреть больше, fluorophores C1 and C2 were significantly enriched in ice, but not in water samples (Fig. The enrichment factor Dc for the salinity-normalized fluorescent maxima of the three fluorophores (Fig. For Fosinopril Sodium (Monopril)- FDA 3, no significant difference was found.



12.10.2020 in 10:34 Роза:

13.10.2020 in 01:22 cocaspass:
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13.10.2020 in 22:21 Олег:
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17.10.2020 in 23:21 presmortwhearos1987:
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